Thermo Scientific™ Pierce™ Human IL-10 ELISA Kits

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: SIL10

HUMAN IL-10 ELISA ST&ARD

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10548893

Quantity Price
1 £ 105.0 / Each
Estimated Shipment date
from Supplier 20-12-2016
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Description and Specification

Specification

Assay Range 15.4 to 600 pg/mL
Description Human IL-10 Standard for ELISA
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Calibrated to NIBSC reference lot 93/722
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 vial
Sensitivity <3 pg/mL
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50 ┬ÁL
Specificity SIL10
Sufficient For ELISA standard curve with replicates
Total Assay Time 3 hrs
Target Natural and recombinant human interleukin-10 (IL-10)

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.