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Thermo Scientific™ Pierce™ Human IL-10 ELISA Kits

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ SIL10

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10548893

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Specifications

Description

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Description & Specifications

Specifications

15.4 to 600 pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV <10%; inter-assay CV <10%
1 vial
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
SIL10
3 hrs
Human IL-10 Standard for ELISA
Calibrated to NIBSC reference lot 93/722
Absorbance at 450nm minus absorbance at 550nm
<3 pg/mL
50 ┬ÁL
ELISA standard curve with replicates
Natural and recombinant human interleukin-10 (IL-10)