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Invitrogen™ eBioscience™ Mouse TNF alpha, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Brand:  Invitrogen™ 34-8321-63

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15598776

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.
Specifications

Specifications

NM_013693
Cytokine Bioassays
The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
SDS-PAGE
10μg
Store at less than or equal to -70°C.
E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF alpha accession # NM_013693
Tumor Necrosis Factor alpha, TNFa TNF-a
Recombinant Protein, Carrier-Free
Mouse
0.5mg/mL
Sterile liquid; 10mM sodium phosphate, pH 7.2, 0.22μm filtered.
TNF alpha
>98%
E. coli
RUO
Less than 0.01ng/μg cytokine as determined by the LAL assay.
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.
Unlabeled
Yes
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