eBioscience™ Mouse TNF alpha, Recombinant Protein, Carrier-Free

Mouse Recombinant Protein

Brand: Affymetrix eBioscience

Manufacturer Part Number: 34-8321-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Product Code. 15508786

Quantity Price
1 £ 317.33 / 100µg
Estimated Shipment date
from Supplier 02-06-2017
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Description and Specification


Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Molecular Weight The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
For Use With (Application) Cytokine Bioassays
Concentration 0.5mg/mL
Name TNF alpha
Cross Reactivity Mouse
Recombinant Yes
Quantity 100μg
Conjugate Unlabeled
Formulation Sterile liquid; 10mM sodium phosphate, pH 7.2, 0.22μm filtered.
Storage Requirements Store at less than or equal to -70°C.
Purification Method SDS-PAGE
Purity >98%
Product Type Recombinant Protein, Carrier-Free
Accession Number NM_013693
Structural Form E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF alpha accession # NM_013693
Gene Symbol Tumor Necrosis Factor alpha, TNFa TNF-a
Biological Activity The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 107 Units/mg.
Regulatory Status RUO
Source E. coli

Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.