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Invitrogen™ IgG4 Human ELISA Kit

For the quantitative detection of human IgG4.

Brand:  Invitrogen™ BMS2095TWO

412.02 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15551637

  • £618.00 / Pack of 2
Estimated Shipment: 09-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human total IgG

Description

Description

The Human ImmunoGlobulin 4 (Hu IgG4) ELISA quantitates Hu IgG4 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG4. Principle of the method The Human IgG4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Several studies have shown that a large population of patients with recurrent respiratory tract infection have low IgG4 concentrations.
Specifications

Specifications

9.4-600 ng/mL
HRP
Plasma, Serum
ELISA
10.2%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
2 x 96 Tests
Plasma, 20 μL; Serum, 20 μL
Immunoglobulin G4
1 hr. 20 min.
0.1 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
4.6%
HRP
RUO
2°C to 8°C
Human
1 hr. 30 min.
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