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Invitrogen™ IgG3 Human ELISA Kit

For the quantitative detection of human IgG3.

Brand:  Invitrogen™ BMS2094TWO

412.02 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15511637

  • £618.00 / Pack of 2
Estimated Shipment: 09-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IgG3

Description

Description

The Human Immunoglobulin G3 (Hu IgG3) ELISA quantitates Hu IgG3 in human serum or plasma. The assay will exclusively recognize both natural and recombinant Hu IgG3. Principle of the method The Human IgG3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

4.7-300 ng/mL
HRP
Plasma, Serum
ELISA
3%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
2 x 96 Tests
Plasma, 10 μL; Serum, 10 μL
Immunoglobulin G2
1 hr. 20 min.
0.21 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
5.6%
HRP
RUO
2°C to 8°C
Human
1 hr. 30 min.
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