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Human IFN gamma, Recombinant Protein, Carrier-Free, Invitrogen™

Recombinant Protein

£137.00

Specifications

Name Human IFN gamma Carrier-Free
Recombinant Recombinant
Conjugate Unlabeled
Purity Affinity chromatography
Purification Method SDS-PAGE
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Product Code Brand Quantity Price Quantity & Availability  
15578776
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Affymetrix eBioscience
34-8319-82
100μg This item is currently unavailable or has been discontinued.
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Description

Description

IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines. Description: Human IFN gamma is an ∽20kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN gamma does not affect its biological activity. Applications Reported: Recombinant human IFN gamma is biologically active and is for bioassay use only. It has been demonstrated to protect A549 cell line against viral infection in culture. For an ELISA standard, please refer to eBioscience Cat. No. 29-8319-65. Applications Tested: The ED50 of this protein, as measured by EMC virus protection assay using A549 cells, is less than or equal to 175 pg/mlL This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg. Source: E. coli expressed amino acids Gln24-Gln166 of mature human IFN gamma accession # NM_000619. Bioactivity: The ED50 of this protein, as measured by EMC virus protection assay using A549 cells, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg. Endotoxin: Less than 0.01ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: The methionylated N-terminal protein migrates as a 16kDa polypeptide on SDS-PAGE. Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.
Specifications

Specifications

Human IFN gamma Carrier-Free
Unlabeled
SDS-PAGE
Cytokine Bioassays
-80°C
Recombinant
Affinity chromatography
E. coli
The methionylated N-terminal protein migrates as a 16kDa polypeptide on SDS-PAGE.