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Thermo Scientific™ Pierce™ Human IFN gamma ELISA Kits

Measure levels of human interferon gamma (IFNg) in human serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ SIFNG

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10157483

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These human IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IFNγ antibody pairs and IFNγ standard only. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interferon gamma (IFNγ)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 25.6 to 1000pg/mL

  • Sensitivity: ≤ 2pg/mL

  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, TNFα or GM-CSF

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Specifications

Specifications

25.6 to 1000 pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
1 vial
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
SIFNG
3.5 hrs
Human IFN gamma Standard for ELISA
Recombinant human IFN gamma from E. coli; Lyophilized powder; contains BSA; Calibrated to NIAID reference lot Gxg-01-902-535
Absorbance at 450nm minus absorbance at 550nm
≤ 2 pg/mL
50 ┬ÁL
ELISA standard curve with replicates
Natural and recombinant human interferon gamma (IFNg)