Thermo Scientific™ Pierce™ Human I-TAC ELISA Kits

Measure levels of human interferon-inducible T-cell alpha chemoattractant in human serum, plasma and culture supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EHITAC

HUMAN ITAC COLORIMETRIC ELISA KIT

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 12341873

Quantity Price
1 £ 430.0 / Each
Estimated Shipment date
from Supplier 14-12-2016
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Description and Specification

Specification

Assay Range 41.0 to 4000pg/mL
Description Human I-TAC ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Precision Intra-assay CV <10%; inter-assay CV <10%
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Sensitivity <5pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50┬ÁL
Specificity No significant cross-reaction with human MIG/CXCL9, IP-10/CXCL10, IL-8, IFNa or mouse ITAC/CXCL11
Sufficient For 96 microplate wells
Total Assay Time 3.5 hr.
Target Natural and recombinant human I-TAC (human interferon-inducible T-cell alpha chemoattractant, also called CXCL11, IP9)

These human I-TAC ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of I-TAC (also known as CXCL11 or IP9) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human I-TAC (human interferon-inducible T-cell alpha chemoattractant, also called CXCL11, IP9)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 41.0 to 4000pg/mL

  • Sensitivity: < 5pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human MIG/CXCL9, IP-10/CXCL10, IL-8, IFNα or mouse ITAC/CXCL11

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.