Invitrogen™ Granzyme B Human ELISA Kit

For the quantitative detection of human Granzyme B.

Product Code	Brand	Quantity
15551517	Invitrogen™ BMS2027TEN	10 x 96 Tests

Description

The Human Granzyme B ELISA quantitates Hu Granzyme B in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme B. Principle of the method The Human Granzyme B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

• Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders
• Not all granzymes enter the target cell, part of them also 'leak' in to the peripheral blood and other biological fluids
• Detectable amounts of granzymes have been found to circulate in healthy volunteers
• These soluble granzymes can be measured by ELISAs

Specifications

• Accession Number: P10144
• Assay Sensitivity: 0.2 pg/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: C11,CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT
• Intraassay CV: 8.5%
• Label or Dye: HRP
• Sample Volume: Serum, 50 μL; Supernatant, 50 μL
• Synonym: Granzyme 2, Fragmentin 2, Human lymphocyte protein
• Test Time: 1 hr. 20 min.

• Assay Range: 0.66-480 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 3002
• Interassay CV: 10.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 2 hr. 45 min.

For Research Use Only