Invitrogen™ eBioscience™ Human Brain-Derived Neurotrophic Factor (BDNF), Recombinant Protein

Human Recombinant Protein

Brand:  Invitrogen™ 14-8366-80

Product Code. 15517536

Description

Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, proBDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation.

Specifications

• Accession Number: NM_001709
• For Use With (Application): Cytokine Bioassay
• Molecular Weight (g/mol): 15.4kDa
• Purification Method: SDS-PAGE
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Biological Activity: The ED50 of this protein, as measured by proliferation of C6 cells, is less than or equal to 100ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 10⁴ Units/mg.
• Conjugate: Unconjugated
• Recombinant: Recombinant

• Concentration: 0.1mg/mL
• Formulation: Sterile liquid; 20mM NaCitrate, 300mM NaCl, 1% BSA, pH 4.0, 0.22μm filtered
• Name: Brain-Derived Neurotrophic Factor (BDNF)
• Quantity: 25μg
• Storage Requirements: Store at less than or equal to -70°C.
• Structural Form: E. coli expressed amino acids His129-Arg247 accession number NM_001709
• Gene Symbol: Brain-Derived Neurotrophic Factor
• Product Type: Recombinant Protein
• Cross Reactivity: Human
• Purity or Quality Grade: ≥97%