eBioscience™ Human Brain-Derived Neurotrophic Factor (BDNF), Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-8366-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15517536

Quantity Price
1 £ 237.85 / 25µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Concentration 0.1mg/mL
Purification Method SDS-PAGE
Storage Requirements Store at less than or equal to -70°C.
Regulatory Status RUO
Gene Symbol Brain-Derived Neurotrophic Factor
Structural Form E. coli expressed amino acids His129-Arg247 accession number NM_001709
Formulation Sterile liquid; 20mM NaCitrate, 300mM NaCl, 1% BSA, pH 4.0, 0.22μm filtered
Cross Reactivity Human
For Use With (Application) Cytokine Bioassays
Molecular Weight 15.4kDa
Product Type Recombinant Protein
Name Brain-Derived Neurotrophic Factor (BDNF)
Accession Number NM_001709
Conjugate Unlabeled
Source E. coli
Endotoxin Concentration Less than 0.01ng/μg cytokine as determined by the LAL assay.
Purity ≥97%
Quantity 25μg
Biological Activity The ED50 of this protein, as measured by proliferation of C6 cells, is less than or equal to 100ng/mL. This corresponds to a specific activity of greater than or equal to 1 x 104 Units/mg.
Recombinant Yes

Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, proBDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation.