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Invitrogen™ Human GM-CSF ELISA Kits

Granulocyte Macrophage Colony Stimulating Factor Recombinant protein SGMCSF detects Granulocyte Macrophage Colony Stimulating Factor in Not Applicable samples, and is validated for Control, ELISA and WB

Brand:  Invitrogen™ EHGMCSF

Code : PN

Additional Details : Weight : 0.45000kg Transport : UN number : 3259 Chem class : 1b Pack group : III

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Product Code. 10147543

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

These human GM-CSF ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of granulocyte macrophage colony-stimulating factor (GM-CSF) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 2pg/mL

  • Reproducibility: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 4 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-6, IL-7, IL-8, IFNγ, TNFα or mouse GM-CSF

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

15.4 to 600pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Human GM-CSF Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 8mL, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human GM-CSF Standard, 2 vials, Standard Diluent, 12mL, Stop Solution, 13mL, Streptavidin-HRP Concentrate, 75µL, TMB Substrate Solution, 13mL
1 plate kit
<2pg/mL
50µL
96 microplate wells
Natural and recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF)
Human GM-CSF ELISA Kit
Complete ELISA kit with coated 96-well plate
Absorbance at 450nm minus absorbance at 550nm
Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with human IL-1a, IL-1ß, IL-2, IL-3, IL-6, IL-7, IL-8, IFNg, TNFa or mouse GM-CSF
4 hr.
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