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Invitrogen™ Fibronectin Human ELISA Kit

For the quantitative detection of human Fibronectin.

£451.00 - £3070.00

Specifications

Accession Number P02751
Assay Range 0.31-20.0 ng/mL
Assay Sensitivity 0.1 ng/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Fibronectin
Biotin-Conjugate anti-human Fibronectin polyclonal antibody
Streptavidin-HRP
Human Fibronectin Standard lyophilized, 40ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15561517
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Invitrogen™
BMS2028
96 Tests
£451.00
96 tests
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Estimated Shipment: 26-03-2024
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15571517
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Invitrogen™
BMS2028TEN
10 x 96 Tests
£3070.00
Pack of 10
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Estimated Shipment: 11-04-2024
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Description

Description

The Human Fibronectin ELISA quantitates Hu Fibronectin in human serum, plasma, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Fibronectin. Principle of the method The Human Fibronectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P02751
0.1 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
CIG,ED-B,FINC,FN,FNZ,GFND,GFND2,LETS,MSF
5.3%
HRP
Plasma, 10 μL; Serum, 10 μL; Supernatant, 50 μL; Urine,50 μL
Cold insoluble globulin, FINC, LETS, MSF, FN
1 hr. 20 min.
0.31-20.0 ng/mL
Biotin
Plasma, Serum, Supernatant, Urine
ELISA
2335
6.7%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
Human
3 hr. 10 min.
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For Research Use Only