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Invitrogen™ FABP2 (intestinal) Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHFABP2

360.01 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.50000kg

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Product Code. 15372098

  • £540.00 / Each
Estimated Shipment: 23-04-2024
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Description

Description

The Human FABP2 (intestinal) ELISA quantitates Hu FABP2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FABP2. Principle of the method The Human FABP2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The intracellular fatty acid-binding proteins belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
Specifications

Specifications

P12104
25 pg/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
2169
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Other Research
2°C to 8°C
Human
4 hr. 45 min.
0.024-100 ng/mL
25pg/mL to 100ng/mL
Biotin
Other Proteins
In this ELISA antibody pair format, no cross-reactivity was observed with rhFABP-1, 3, 5, 6, 7, 8 and 9.
Human
Colorimetric Microplate Reader
FABP2
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
FABPI, I-FABP
1 hr. 20 min.
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