eBioscience™ Annexin V, FITC, Recombinant Protein

Recombinant Protein

Brand: eBioscience™

Manufacturer Part Number: BMS306FI/300

300TEST Annexin V-FITC Recombinant Protein

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg

Product Code. 15541917

Quantity Price
1 £ 198.17000000000002 / 300 tests
Estimated Shipment date
from Supplier 28-03-2017
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Description and Specification


Molecular Weight 35.8kDa
Name Annexin V
Purification Method SDS-gel electrophoresis and reverse phase HPLC
Purity >89%
Quantity 300 tests
Recombinant Yes
Source E. coli
Storage Requirements Annexin V-FITC is stable at 2-8deg;C as indicated on the label. Do not freeze!
Regulatory Status RUO
Structural Form E. Coli
Gene Symbol Lipocortin V, Endonexin II, Calphobindin I
Conjugate FITC
Biological Activity Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
Product Type Recombinant Protein
For Use With (Application) Flow Cytometric Analysis
Formulation Solution containing 50mM TRIS, 100mM NaCl, 1% BSA, 0.02% Sodium Azide, pH 7.4

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bindto phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantlylocated along the cytosolic side of the plasma membrane. Upon initiation of apoptosis,PS loses its asymmetric distribution in the phospholipid bilayer and translocates tothe extracellular membrane, which is detectable with fluorescently labeled AnnexinV. In early stages of apoptosis, the plasma membrane excludes viability dyes such aspropidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining(PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss ofcell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptakeof PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identifyapoptotic stages by flow cytometry.