Invitrogen™ Cu/ZnSOD Human ELISA Kit

For the quantitative detection of human Cu/ZnSOD.

Brand:  Invitrogen™ BMS222TEN

Product Code. 15561737

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cu/ZnSOD
HRP-Conjugate anti-human Cu/ZnSOD monoclonal antibody
Human Cu/ZnSOD Standard, 5ng/mL
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Phosphate Buffered Saline Concentrate (PBS) 20x
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

The Human Cu/Zn Superoxide Dismutase(Hu Cu/ZnSOD)) ELISA quantitates Hu Cu/ZnSOD in human serum, plasma, amniotic fluid, urine, fetal umbilical vein blood, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Cu/ZnSOD. Principle of the method The Human Cu/ZnSOD solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein,  acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

Specifications

• Accession Number: P00441
• Assay Sensitivity: 0.04 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: ALS,ALS1,HEL-S-44,IPOA,SOD,hSod1,homodimer
• Intraassay CV: 5.1%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 1 hr. 10 min.

• Assay Range: 0.08-5.0 ng/mL
• Conjugate: HRP
• Sample Type: Amniotic Fluid, Fetal Umbilical Vein Blood, Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 6647
• Interassay CV: 5.8%
• Kit Contents: Controls, Adhesive Plate Covers, Sample Diluent, Standard, Sample Diluent, Wash Buffer, Chromogen, Wash Buffer, Chromogen, Stop Solution
• Quantity: 10 x 96 Tests
• Sample Volume: Amniotic Fluid, 10 μL; Fetal Umbilical Vein Blood,10 μL; Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL; Urine,10 μL
• Synonym: Cu/Zn Superoxide Dismutase
• Test Time: 1 hr. 20 min.

For Research Use Only