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Invitrogen™ Caspase 9 Human ELISA Kit

For the quantitative detection of human Caspase-9.

Brand:  Invitrogen™ BMS2025

300.68 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15531517

  • £451.00 / 96 tests
Estimated Shipment: 22-04-2024
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-9
Anti-human Caspase-9 polyclonal Detection Antibody (rabbit)
Anti-rabbit-IgG-HRP
Human Caspase-9 Standard lyophilized, 200ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

Description

The Human Caspase 9 ELISA quantitates Hu Caspase 9 in human serum, cell lysate or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Caspase 9. Principle of the method The Human Caspase 9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 that contains a CARD domain. Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Further, Caspase 9 is involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Caspase 9 promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner, and proteolytically cleaves poly(ADP- ribose) polymerase (PARP). Caspase 9 is expressed in variety of human tissues. An isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. Mutations in Caspase 9 affect brain development. In mice, insufficient Caspase 9 have resulted in abnormal cerebrum development and neuron development. In humans, dysfunctional Caspase 9 expression vary from tissue to tissue. Low levels of Caspase 9 may play a role in cancer development and neurodegenerative diseases such as Alzheimer’s Disease (AD).
Specifications

Specifications

P55211
0.4 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
APAF-3,APAF3,ICE-LAP6,MCH6,PPP1R56
6.6%
HRP
RUO
2°C to 8°C
Human
3 hr. 10 min.
1.6-100 ng/mL
HRP
Cell Lysates, Serum, Supernatant
ELISA
842
9%
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Assay Buffer concentrate, Detection Antibody, Lysis Buffer, Wash Buffer, Wash Buffer, Stop Solution, Stop Solution
96 Tests
Cell Lysate,50 μL; Serum, 50 μL; Supernatant, 50 μL
Apoptotic protease MCH-6, APAF-3, ICE-LAP6
1 hr. 20 min.
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