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Invitrogen™ Caspase 9 Human ELISA Kit

For the quantitative detection of human Caspase-9.

£451.00 - £3070.00

Specifications

Accession Number P55211
Assay Range 1.6-100 ng/mL
Assay Sensitivity 0.4 ng/mL
Conjugate HRP
Product Type ELISA Kit
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Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-9
Anti-human Caspase-9 polyclonal Detection Antibody (rabbit)
Anti-rabbit-IgG-HRP
Human Caspase-9 Standard lyophilized, 200ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

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Product Code Brand Quantity Price Quantity & Availability  
15531517
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Invitrogen™
BMS2025
96 Tests
£451.00
96 tests
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15541517
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Invitrogen™
BMS2025TEN
10 x 96 Tests
£3070.00
Pack of 10
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Estimated Shipment: 23-04-2024
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Description

Description

The Human Caspase 9 ELISA quantitates Hu Caspase 9 in human serum, cell lysate or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Caspase 9. Principle of the method The Human Caspase 9 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
Specifications

Specifications

P55211
0.4 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
APAF-3,APAF3,ICE-LAP6,MCH6,PPP1R56
6.6%
HRP
Cell Lysate,50 μL; Serum, 50 μL; Supernatant, 50 μL
Apoptotic protease MCH-6, APAF-3, ICE-LAP6
1 hr. 20 min.
1.6-100 ng/mL
HRP
Cell Lysates, Serum, Supernatant
ELISA
842
9%
Pre-coated 96 well plate, Standard, Standard, Assay Buffer concentrate, Detection Antibody, anti-rabbit HRP conjugate, anti-rabbit HRP conjugate, Wash Buffer, Wash Buffer, Chromogen, Stop Solution
RUO
2°C to 8°C
Human
3 hr. 10 min.
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Product Certifications

For Research Use Only