Invitrogen™ Complement C3a Human ELISA Kit

For the quantitative detection of human C3a.

Brand:  Invitrogen™ BMS2089TEN

Product Code. 15517925

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human C3a

Description

The Human Complement C3a (Hu C3a) ELISA quantitates Hu C3a in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C3a. Principle of the method The Human C3a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.

Specifications

• Assay Sensitivity: 0.07ng/mL
• Sample Type: Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
• For Use With (Application): ELISA
• Synonym: complement component 3, ASP, C3a, C3b, C3, Acylation stimulating protein

• Conjugate: Biotin
• Target Species: Human
• Quantity: 10 x 96 Tests
• Target: C3a

For Research Use Only