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Invitrogen™ C1q Human ELISA Kit

For the quantitative detection of human C1q.

Brand:  Invitrogen™ BMS2099TWO

412.02 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15531657

  • £618.00 / Pack of 2
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Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human C1q

Description

Description

The Human C1q ELISA quantitates Hu C1q in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C1q. Principle of the method The Human C1q solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1.
Specifications

Specifications

P02745
0.08 ng/mL
ELISA Kit
Human
Colorimetric Microplate Reader
C1QA
4.7%
HRP
RUO
2°C to 8°C
Human
4 hr. 30 min.
0.9-60 ng/mL
Biotin
Plasma, Serum, Supernatant
ELISA
712
4.2%
Pre-coated 96 well plate, Adhesive Plate Covers, Sample Diluent, Sample Diluent, Biotinylated Detection Antibody, Biotinylated Detection Antibody, Wash Buffer, Lysis Buffer, Lysis Buffer, Stop Solution, Stop Solution
2 x 96 Tests
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
complement component 1, q subcomponent, A chain el, complement component 1, q subcomponent, alpha poly
1 hr. 20 min.
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