Invitrogen™ Anti-IFN alpha Antibody Human ELISA Kit

For the quantitative detection of human anti-IFN-alpha.

Brand:  Invitrogen™ BMS217TEN

Product Code. 15541707

Includes: Aluminum pouch(es) with a Microwell Plate coated with recombinant human IFN-alpha
HRP-Conjugate human IFN-alpha protein
Human anti-IFN-alpha Standard, 200ng/mL
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films

Description

The Human Interferon alpha Antibody (Hu anti-IFNα) ELISA quantitates Hu anti-IFNα in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu anti-IFNα. Principle of the method The Human anti-IFNα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: P01562,P01563
• Assay Sensitivity: 1.4 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IFL,IFN,IFN-ALPHA,IFN-alphaD3,IFNA@,IFNA1,IFN-alphaA,IFNAB,INFA2,IFNA2
• Intraassay CV: 3.3%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 3.15-200 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant, Urine
• For Use With (Application): ELISA
• Gene ID (Entrez): 3439, 3440
• Interassay CV: 4.1%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 20 μL; Urine,20 μL
• Synonym: Anti-alpha interferon, IFN leukocytic, LeIF D
• Test Time: 1 hr. 20 min.

For Research Use Only