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Invitrogen™ Angiopoietin 1 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHANGPT1

Code : NEW

Additional Details : Weight : 0.33000kg

Product Code. 15484893

  • £424.00 / 1 set


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Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

Description

The Human Angiopoietin 1 (Hu APO1) ELISA quantitates Hu APO1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu APO1. Principle of the method The Human APO1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential Fibrinogen-like domain at the carboxy-terminus and coiled-coil regions in the amino-terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling.
Specifications

Specifications

Q15389
ELISA Kit
30pg/mL to 18000pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 33 μL|Serum, 33 μL|Supernatant, 100 μL
2°C to 8°C
1 hr. 20 min.
Human
Angiogenesis
<12%
96 Tests
Supernatant
30 pg/mL
Colorimetric Microplate Reader
HRP
74.07-18,000 pg/mL
Biotin
284
RUO
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
96 assays
4 hr. 45 min.
Kinase & Inhibitors
AGP1, AGPT, ANG1
<10%
Human
Solid-phase sandwich ELISA
AGP1,AGPT,ANG1
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