eBioscience™ SAFE™ Human TNF alpha, Recombinant Protein

Human Recombinant Protein

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 68-8786-82

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Product Code. 15510637

Quantity Price
1 £ 158.36 / 100µg
Estimated Shipment date
from Supplier 09-05-2017
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Description and Specification

Specification

For Use With (Application) Functional Assays
Conjugate Unlabeled
Biological Activity The ED50 of this protein, as measured by cytolystic activity of actinomycin-D sensitized L929 cells, is less than or equal to 0.4ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 106 Units/mg.
Endotoxin Concentration Less than 0.005ng/μg (0.05 EU/μg) as measured by turbitidity assay
Purity ≥95%
Storage Requirements The lyophilized powder can be stored at less than or equal to -20°C. (Short term storage at 2-8°C). Reconstitute in sterile PBS to a concentration no less than 100μg/mL. Once reconstituted, protein should be aliquoted and stored at -20°C or lower. For best stability add 0.1% BSA before freezing aliquots
Regulatory Status RUO
Molecular Weight 24kDa
Gene Symbol Tumor Necrosis Factor alpha
Product Type Recombinant Protein
Formulation Sterile lyophilized (PBS buffer pH 7.2)
Source Plant
Name TNF alpha
Purification Method SDS-PAGE
Recombinant Yes
Cross Reactivity Human
Structural Form Plant expressed 158 amino acids (Val77-Leu233) with 16 amino acid Histidine tag. Accession P01375
Quantity 100μg

This SAFE (Serum and Animal Free, low Endotoxin) protein has been made in plants with no exposure to animal protein during expression or purification. This animal free product has very low levels of endotoxin.

Human TNF alpha is a memeber of the TNF ligand superfamily produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 24 kDa form of TNF alpha is expressed as a membrane bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed. Human and mouse TNF alpha conservation is about 79%, Human protein is functional on mouse, although with slightly reduced activity.