HSP70 Monoclonal Antibody (1B2hsp70), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9115-80

Product Code. 15597576

Description

Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75kDa protein that is found within the mitochondria.

Description: The monoclonal antibody 1B2hsp70 recognizes HSP (heat shock protein) 70. Hsps are highly conserved molecular chaperones that associate with proteins to stabilize or aid in the folding of new proteins or denatured proteins following chemical or physical stress. Hsp expression is both constitutive and inducible depending on the cell and its surrounding conditions. Hsps have been known to be upregulated in conditions such as cancer, ischemic injury, inflammatory, and demyelinating disease.This antibody recognizes the Hsp70 family member 1A found in the cytoplasm and nucleus of numerous cell types. Given the high degree of conservation of this protein across species, the 1B2hsp70 clone is expected to recognize canine and monkey HSP70 1a.Applications Reported: This 1B2hsp70 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.Applications Tested: This 1B2hsp70 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded tissue using both low and high pH antigen retrieval at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: HSP70
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P0DMV8
• Gene Symbols: HSPA1A
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3303
• Content And Storage: Store at 2-8°C.
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: 1B2hsp70
• Conjugate: Unconjugated
• Gene: HSPA1A
• Gene Alias: HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSP70-1/HSP70-2, HSP70.1/HSP70.2, dnaK-type molecular chaperone HSP70-1, heat shock 70kDa protein 1/2, heat shock 70kDa protein 1A/1B, heat shock 70kD protein 1A, heat shock-induced protein
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.