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HLA-G Mouse anti-Human, Clone: 87G, eBioscience™

Mouse Monoclonal Antibody

£51.00 - £79.75

Specifications

Antigen HLA-G
Clone 87G
Target Species Human
Conjugate Unconjugated
Isotype IgG2a κ
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15228277
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Affymetrix eBioscience
14-9957-80
25 μg
£51.00
25µg
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Estimated Shipment: 30-06-2022
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15238277
View Documents Promotion Details
Affymetrix eBioscience
14-9957-82
100 μg
£79.75
100µg
View alternative products
Save Up to 
»
Estimated Shipment: 30-06-2022
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The monoclonal antibody 87G recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses. HLA-G has seven reported isoforms. The antibody 87G recognizes both HLA-G1 and the solube HLA-G5. Expression of HLA-G is found primarily in fetal trophoblast cells as they invade the maternal decidua thereby protecting the fetus from the maternal immune system. Like the highly mitotic trophoblast, abundant HLA-G protein expression has been identified in some tumors, including melanoma, breast carcinoma and renal carcinoma as well as CLL, AML and B-CLL. Some expression has also been found in pancreatic islets, erythroid and endothelial progenitors and the adult thymic medulla.HLA-G+ CD4 or CD8 cells have been identified in normal human peripheral blood and are thought to act as regulatory cells in that they are hypoproliferative with a unique cytokine profile differing from Tregs. The receptors for HLA-G are CD85j/ILT2, CD85d/ILT4, and CD158. Recent studies have shown a role for HLA-G in tolerance and maintece of transplanted organs. Applications Reported: This 87G antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applications Tested: This 87G antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test.

A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Specifications

Specifications

HLA-G
Human
IgG2a κ
Primary
Antibody
RUO
87G
Unconjugated
Flow Cytometry, Immunohistochemistry (Frozen)
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
SDS
Product Certifications

For Research Use Only.