HLA-E, PE, clone: 3D12HLA-E, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-9953-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.Applications Reported: This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12HLA-E antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|