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HLA-E, PE, clone: 3D12HLA-E, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9953-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15517286

  • £94.97 / 25 tests


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.Applications Reported: This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12HLA-E antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

HLA-E
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P13747
DAQB-90C11.16, FLJ26655, HLAA
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
3D12HLA-E
PE
HLA-E
Conjugated
HLA-E
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3133
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