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HLA-E, PE, clone: 3D12HLA-E, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-9953-42
Code : NEW
Additional Details : Weight : 0.23750kg
Description
Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.Applications Reported: This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12HLA-E antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
HLA-E | |
Monoclonal | |
5μl/Test | |
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P13747 | |
DAQB-90C11.16, FLJ26655, HLAA | |
Murine | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Human |
Flow Cytometry | |
3D12HLA-E | |
PE | |
HLA-E | |
Conjugated | |
HLA-E | |
IgG1, kappa | |
100 Tests | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
3133 |
For Research Use Only.