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HLA-E, Functional Grade, clone: 3D12HLA-E, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9953-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15557836

  • £143.29 / 100µg


EU Stock 1
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Description

Description

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.Applications Reported: This 3D12HLA-E antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12HLA-E antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

HLA-E
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P13747
DAQB-90C11.16, FLJ26655, HLAA
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Functional Assay
3D12HLA-E
Functional Grade
HLA-E
Purified
HLA-E
IgG1, kappa
100μg
Store at 2-8°C.
Primary
3133
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