HLA-DR Mouse anti-Human, V500, Clone: G46-6, BD

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15850869	BD Biosciences 561225	25 Tests
13442109	BD Biosciences 561224	100 Tests

Description

The G46-6 monoclonal antibody specifically binds to HLA-DR, a major histocompatibility complex (MHC) class II antigen. HLA-DR antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. HLA-DR is a transmembrane heterodimeric glycoprotein composed of an α chain (36 kDa) and a β subunit (27 kDa) expressed primarily on antigen presenting cells: B cells, dendritic cells, monocytes, macrophages, and thymic epithelial cells. HLA-DR is also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4-positive T cells.

When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls. Due to spectral differences between cells and beadsin this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents.Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.Different V500 reagents (e.g. CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Specifications

• Antigen: HLA-DR
• Applications: Flow Cytometry
• Conjugate: Violet Fluor 500 (V500)
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing protein stabilizer, glycerol and ≤0.09% sodium azide.
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Clone: G46-6
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Isotype: IgG2a κ
• Purification Method: Affinity Purified
• Description: MHC class II antigen; HLA class II histocompatibility antigen

For Research Use Only.