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HLA-DR, eVolve 605, clone: LN3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 83-9956-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15500767

  • £87.13 / 25 tests


Estimated Shipment date
from Supplier 27-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes.Applications Reported: This LN3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

HLA-DR
LN3
eVolve 605
Conjugated
IgG2b
RUO
Primary
Human
Flow Cytometry
5μL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Monoclonal
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