missing translation for 'onlineSavingsMsg'
Learn More

HLA-ABC, PE-Cyanine5, clone: W6/32, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-9983-42

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15567766

  • £162.21 / 100 tests


EU Stock 1
Add to basket

Description

Description

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43kDa and 11-12kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.Applications Reported: The W6/32 antibody has been reported for use in flow cytometric analysis.Applications Tested: This W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

HLA-ABC
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P05534
H-2K, H-2K(d), H2-K, MGC184092, MGC7052
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
W6/32
PE-Cyanine5
HLA-A
Conjugated
HLA-A
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3105
Videos

For Research Use Only.