HLA-ABC, clone: W6/32, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9983-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43kDa and 11-12kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.Applications Tested: The W6/32 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|H-2K, H-2K(d), H2-K, MGC184092, MGC7052|
|Flow CytometryþImmunohistochemistry (Frozen)þImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.