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HLA-ABC, clone: W6/32, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9983-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15298307

  • £62.67 / 100µg


EU Stock 2
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Description

Description

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43kDa and 11-12kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey.Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.Applications Tested: The W6/32 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

HLA-ABC
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P05534
H-2K, H-2K(d), H2-K, MGC184092, MGC7052
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Immunoprecipitation
W6/32
Unconjugated
HLA-A
Purified
HLA-A
IgG2a, kappa
100μg
Store at 2-8°C.
Primary
3105
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