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HLA-ABC Mouse anti-Human, APC, Clone: W6/32, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-9983-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15578286

  • £74.00 / 25 tests

Estimated Shipment
02-02-2021

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Description

Description

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. maligt cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.
Spécification

Spécification

HLA-ABC
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P05534
H-2K, H-2K(d), H2-K, MGC184092, MGC7052
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
W6/32
APC
HLA-A
Liquid
HLA-A
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3105
Vidéos
FDS
Documents

For Research Use Only.