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Cytiva HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

£171.00 - £7181.50

Specifications

Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C
Particle Size 45 to 165 μm
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Products 4
Product Code Brand Quantity Flow Rate Price Quantity & Availability  
Product Code Brand Quantity Flow Rate Price Quantity & Availability  
11723219
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Cytiva
17-5286-01
5 x 5 mL <20 mL/min.
£582.00
Pack of 5
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10685355
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Cytiva
11-0004-58
60 mL <4 mL/min.
£171.00
Pack of 5
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11800083
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Cytiva
17-5286-02
100 x 5 mL <20 mL/min. This item is currently unavailable or has been discontinued.
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11515674
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Cytiva
11-0004-59
100 x 1 mL <4 mL/min. This item is currently unavailable or has been discontinued.
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Description

Description

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Specifications

Polypropylene
6% Cross-linked Agarose
45 to 165 μm
3 to 12
4°C to 30°C
Pre-packed Column
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