Thermo Scientific™ HisPur™ Ni-NTA Magnetic Beads
Perform affinity purification of His-tagged fusion proteins in manual or automated formats with the high capacity nickel-IMAC Thermo Scientific™ HisPur Ni-NTA Magnetic Beads.
Brand: Thermo Scientific™ 88831
Code : PN
Additional Details : Weight : 0.45360kg
DescriptionThe blocked magnetic bead surface is derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) beads provide high binding capacity with very low background. The HisPur Ni–NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific™ KingFisher Instruments for high–throughput needs.
High capacity – equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers
Low nonspecific binding – the bead surface is pre-blocked and the protocol provides optimized buffers for purification
Fast – protocol is completed in 1 hour
Scalable – process microliter to milliliter sample volumes
Versatile – purify proteins using native or denaturing conditions
Reagent compatible – can be used with common cell lysis reagents and a variety of buffer additives
Multiple formats – protein coupling to the beads and downstream applications can be performed both manually and on an automated platform (e.g., Thermo Scientific™ KingFisher Instruments)
|HisPur Ni-NTA Magnetic Beads|
|Nickel on nitrilotriacetic acid covalently coupled to blocked superparamagnetic beads; supplied at 12.5mg/mL in 20% ethanol|
|Binding at least 500µg green fluorescent protein (GFP)/mL of bead|