Thermo Scientific™ HisPur™ Cobalt Purification Kit, 3 mL

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Brand:  Thermo Scientific™ 90092

Product Code. 11894201

Description

Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.
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HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co²⁺)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni²⁺chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.

Highlights:

• High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions

• Low metal leaching – no metal contamination in eluted histidine-tagged protein sample

• Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives

• Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates

• Cost effective – resin can be reused several times or discarded after single use

• Superior – performs better than other commercially available IMAC Resins

Available Formats:

• Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
• Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
• Purification Kits – complete kits in all three column sizes
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
• 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
• Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin

Includes:

Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer

Specifications

• Capacity (Metric): 1 mL/Column
• Content And Storage: Sufficient For: Purifying ∼30 mg of His-tagged protein per column
  • HisPur Cobalt Spin Columns, 3 mL, 5 columns
  • Equilibration/Wash Buffer, 225 mL
  • Elution Buffer, 75 mL
  
  Store at 4°C.
• Quantity: 5 Column Kit
• Type: Protein Purification System
• Sufficient For: 2 mg of His-Tagged Protein per column
• Column Type: Affinity, Agarose Resin

• Description: HisPur Cobalt Spin Purification Kit, 3mL
• Product Line: HisPur™
• Target: His-Tagged Protein
• Stationary Phase: Metal Chelate, Divalent Cobalt
• Format: Spin Column

Safety and Handling

Product Identifier

• HisPur Cobalt Purification Kit, 3 mL

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Reproductive toxicity Category 1B

Hazard Statement

• H226-Flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H360-May damage fertility or the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Ethanol, 1-Imidazole, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.