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Thermo Scientific™ HisPur™ Cobalt Chromatography Cartridges, 1 mL

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Brand:  Thermo Scientific™ 90093

110.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : PN

Additional Details : CAS Number : 24937-79-9 Weight : 0.80000kg


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Product Code. 11804211

  • £165.00 / Pack of 5
Estimated Shipment: 12-04-2024
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Description

Description

Thermo Scientific™ HisPur Cobalt Resin is an immobilized metal chelate affinity (IMAC) support for specific, fast and gentle His-tagged purification.
Additional Information and Online Ordering »

HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.

Highlights:

  • High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions

  • Low metal leaching – no metal contamination in eluted histidine-tagged protein sample

  • Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives

  • Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates

  • Cost effective – resin can be reused several times or discarded after single use

  • Superior – performs better than other commercially available IMAC Resins

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
  • Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin

Includes:

Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer

Specifications

Specifications

1 mL
Store between 4°C and 8°C.
HisPur™
His-Tagged Protein
Metal Chelate, Divalent Cobalt
HisPur Cobalt Chromatography Cartridges, 1mL
Liquid Suspension
5 Cartridges
Chromatography Cartridge
Affinity, Agarose Resin
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Product Suggestions

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Safety and Handling

Safety and Handling

Product Identifier
  • HisPur Cobalt Chromatography Cartridges

Signal Word
  • Danger

Hazard Category
  • Carcinogenicity Category 1B
  • Reproductive toxicity Category 1
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1

Hazard Statement
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H350-May cause cancer.
  • H360FD-May damage fertility. May damage the unborn child.

Precautionary Statement
  • P101-If medical advice is needed, have product container or label at hand.
  • P102-Keep out of reach of children.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol, cobalt chloride

SDS
Documents

Documents

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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.