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Thermo Scientific™ HisPur™ Cobalt Resin

Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.

Brand:  Thermo Scientific™ 89964

Code : PN

Additional Details : Weight : 1.81440kg Transport : UN number : 1170 Chem class : 3 Pack group : II


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Product Code. 10751375

  • £76.75 / 10mL


EU Stock 1
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Description

Description

HisPur Cobalt Resins, Columns and Kits use a tetradentate chelating resin charged with divalent cobalt (Co2+)to enable specific, fast and gentle purification of histidine-tagged fusion proteins from a variety of sample types. HisPur Cobalt Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high throughput needs. With these options, high-quality His-tag protein purification is possible at nearly any scale. HisPur Cobalt Resin is superior to traditional nickel-based immobilized metal affinity chromatography (IMAC) systems. Compared to Ni-IDA and tetradentate Ni2+chelate resins, HisPur Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Use HisPur Columns and Cartridges to obtain high-purity His-tagged proteins with no metal contamination.

Highlights:

  • High purity – obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions

  • Low metal leaching – no metal contamination in eluted histidine-tagged protein sample

  • Versatility – purify proteins under native or denaturing conditions; compatible with cell lysis reagents and a variety of buffer additives

  • Flexibility – available as bulk resin or predispensed columns, chromatography columns and 96-well filter plates

  • Cost effective – resin can be reused several times or discarded after single use

  • Superior – performs better than other commercially available IMAC Resins

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations
  • Bulk and Custom – let us know your needs for bulk HisPur Cobalt Resin

Includes:

Kits contain HisPur Cobalt Columns, equilibration/wash buffer and elution buffer

Specifications

Specifications

HisPur Cobalt Resin
10mL
Agarose
Cobalt (IMAC) on crosslinked 6% beaded agarose, slurried in 20% ethyl alcohol
Binding >10mg of His-tagged protein per mL of resin
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Safety and Handling

Safety and Handling

Product Identifier
  • HisPur Cobalt Resin

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Carcinogenicity Category 1B
  • Reproductive toxicity Category 1B
  • LONG-TERM AQUATIC HAZARD Chronic 2

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H350-May cause cancer.
  • H360F-May damage fertility.
  • H411-Toxic to aquatic life with long lasting effects.
  • EUH208-Contains A§. May produce an allergic reaction.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/A§/ equipment.
  • P273-Avoid release to the environment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to A§.

Supplemental information
  • MIXTURE LIST-Contains : Cobalt chloride(CoCl2), hexahydrate

Documentation