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6x-His Tag, clone: HIS.H8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-6657-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15547466

  • £160.59 / 100µg


EU Stock 3
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Description

Description

Description: This HIS.H8 monoclonal antibody reacts with the 6X polyhistidine (6X His) tag. The H-H-H-H-H-H motif is frequently fused to the N- or C-terminus of proteins to facilitate purification (e.g., nickel affinity chromatography and immunoprecipitation) and/or detection. This antibody recognizes the 6X His tag anywhere within the protein of interest (i.e., N-terminus, C-terminus, or internal).Applications Reported: This HIS.H8 antibody has been reported for use in immunoprecipitation and western blotting.Applications Tested: This HIS.H8 antibody has been tested by immunoblotting of reduced cell lysate expressing a His-tagged protein at less than or equal to 1 ug/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

His Tag
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
His Tag, Histidine Tag, Histidine Epitope Tag, 6X His eptope tag, 6His tag, 6X His
IgG2b
100μg
Store at 2-8°C.
Primary
Murine
ImmunoprecipitationþWestern Blot
HIS.H8
Unconjugated
Purified
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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