6x-His Tag, clone: HIS.H8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-6657-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This HIS.H8 monoclonal antibody reacts with the 6X polyhistidine (6X His) tag. The H-H-H-H-H-H motif is frequently fused to the N- or C-terminus of proteins to facilitate purification (e.g., nickel affinity chromatography and immunoprecipitation) and/or detection. This antibody recognizes the 6X His tag anywhere within the protein of interest (i.e., N-terminus, C-terminus, or internal).Applications Reported: This HIS.H8 antibody has been reported for use in immunoprecipitation and western blotting.Applications Tested: This HIS.H8 antibody has been tested by immunoblotting of reduced cell lysate expressing a His-tagged protein at less than or equal to 1 ug/ mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|His Tag, Histidine Tag, Histidine Epitope Tag, 6X His eptope tag, 6His tag, 6X His|
|Store at 2-8°C.|
For Research Use Only.