High Endothelial Venule Marker Rat anti-Human, Mouse, Alexa Fluor 488, Clone: MECA-79, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 53-6036-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15372050

Quantity Price
1 £ 84.99 / 25µg
Estimated Shipment date
from Supplier 28-03-2017
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Description and Specification

Specification

Monoclonal or Polyclonal Monoclonal
Regulatory Status RUO
Format Conjugated
Host Species Rat
Applications Flow Cytometry
Applications Immunohistochemistry (Formalin/Paraffin)
Gene Alias HEV, PNAd, MECA-79
Quantity 25μg
Species Reactivity Human, Mouse
Concentration 0.5mg/mL
Clone MECA-79
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Isotype IgM
Antigen High Endothelial Venule Marker
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Primary or Secondary Primary
Conjugate Alexa Fluor 488

This MECA-79 monoclonal antibody recognizes a sulfated carbohydrate epitope present on human and mouse high endothelial venules (HEVs) called peripheral lymph node addressin (PNAd). This glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer’s patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues.

The MECA-79 antibody has been reported to inhibit CD62L (L-selectin)-dependent lymphocyte emigration to HEVs and adhesion in lymph nodes and tonsils.