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HIF-1 alpha, clone: ESEE122, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9100-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15258147

  • £69.66 / 25µg


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Description

Description

Description: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the constitutively expressed beta form, also called the aryl hydrocarbon receptor nuclear translocator (ARNT). Both alpha and beta subunits of HIF-1 contain basic helix-loop-helix motifs and function to activate transcription of genes in response to reduced oxygen levels. Under normoxic conditions, HIF-1 alpha is rapidly degraded, while HIF-1 alpha expression is induced and degradation inhibited under hypoxic conditions. HIF-1 alpha can be found in the cytoplasm and/or in the nucleus under normoxic conditions, but translocates to the nucleus under hypoxic conditions. HIF-1 is upregulated in several cancer types and functions to control genes involved in angiogenesis, cell survival and T cell development. Recent studies have shown the importance of HIF-1 alpha in balancing Th17 and Treg development.This ESEE122 antibody has also been reported to cross-react with bovine, rat, and mouse hypoxia inducible factor-1 alpha.Applications Reported: This ESEE122 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This ESEE122 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta at less than or equal to 10 ug/mL using low pH antigen retrieval buffer. This ESEE122 antibody has been tested by immunocytochemistry on fixed and permeabilized Hela cells at less than or equal to 10 ug/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

HIF-1
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9XTA5, Q16665, O35800, Q61221
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
BovineþHumanþMurineþRat
ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry
ESEE122
Unconjugated
HIF1A
Purified
HIF1A
IgG1, kappa
25μg
Store at 2-8°C.
Primary
3091
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