Thermo Scientific™ HIF-1 alpha ELISA Kit, Human

Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EHIF1A

UNSPSC: 41116126

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15398184

Quantity Price
1 £ 398.0 / Each
EU Stock 3
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Description and Specification

Specification

Cross Reactivity Mouse and rat HIF-1A
Description HIF-1 Alpha ELISA Kit, 96 Assays, 1 Kit, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Reagents
Regulatory Status For research use only. Not for use in diagnostic procedures
Measurement Absorbance at 450nm minus absorbance at 550nm
Assay Range 81.92 to 20,000pg/mL
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Packaging Quantity 1 Kit(s)
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Sensitivity ≤30pg/mL
Formulation Solid
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Assays 96
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Quantity 1 Kit(s)
Quantity 96 assays
Research Discipline Immunology
Species Human
Sufficient For 96 Determinations
Host Species Human
Precision Intra-assay CV <10%; inter-assay CV <10%
Sample Size 50μL
Storage Requirements 2 to 8°C
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Conjugate Streptavidin-HRP
Product Type HIF-1 Alpha ELISA Kit, Human
Size 96 Assay(s)
Measuring Range 450 to 550nm
Sample Volume 50μL
Total Assay Time 3.5 hr.
Detection Method Colorimetric
For Use With (Application) Protein assays and analysis
Substrate Type TMB
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Test Time 3.5 hr.
Protein Family Transcription Factors