Thermo Scientific™ HIF-1 alpha ELISA Kit, Human

Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EHIF1A5

UNSPSC: 12352200

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15318194

Quantity Price
1 £ 1384.0 / Pack of 5
Estimated Shipment date
from Supplier 07-04-2017
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Description and Specification

Specification

Research Discipline Immunology
Detection Method Colorimetric
For Use With (Application) Protein assays and analysis
Formulation Solid
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 Kit(s)
Quantity 480 assays
Protein Family Transcription Factors
Description HIF-1 Alpha ELISA Kit, 480 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Test Time 3.5 hr.
Sensitivity ≤30pg/mL
Conjugate Streptavidin-HRP
Measuring Range 450 to 550nm
Product Type HIF-1 Alpha ELISA Kit, Human
Assay Range 81.92 to 20,000pg/mL
Sample Volume 50μL
Regulatory Status For research use only. Not for use in diagnostic procedures
Sample Size 50μL
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Precision Intra-assay CV <10%; inter-assay CV <10%
Total Assay Time 3.5 hr.
Storage Requirements 2 to 8°C
Sufficient For 480 Determinations
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Substrate Type TMB
Cross Reactivity Mouse and rat HIF-1A
Species Human
Host Species Human
Packaging Quantity 1 Kit(s)
Size 480 Assay(s)
Assays 480