Thermo Scientific™ HIF-1 alpha ELISA Kit, Human

Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: EHIF1A2

UNSPSC: 12352200

Code: NEW

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15308194

Quantity Price
1 £ 576.0 / Pack of 2
EU Stock 1
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Description and Specification

Specification

Protein Family Transcription Factors
Substrate Type TMB
Assays 192
Assay Range 81.92 to 20,000pg/mL
Measuring Range 450 to 550nm
Packaging Quantity 1 Kit(s)
Sensitivity ≤30pg/mL
Sample Volume 50μL
Size 192 Assay(s)
Host Species Human
Research Discipline Immunology
Measurement Absorbance at 450nm minus absorbance at 550nm
Sample Size 50μL
Test Time 3.5 hr.
Regulatory Status For research use only. Not for use in diagnostic procedures
Includes Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Sample Type Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Storage Requirements 2 to 8°C
Sufficient For 192 Determinations
Cross Reactivity Mouse and rat HIF-1A
For Use With (Application) Protein assays and analysis
Total Assay Time 3.5 hr.
Product Type HIF-1 Alpha ELISA Kit, Human
Formulation Solid
Precision Intra-assay CV <10%; inter-assay CV <10%
Specificity Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
Target Natural and recombinant human hypoxia-inducible factor 1-alpha (HIF1A)
Detection Method Colorimetric
Description HIF-1 Alpha ELISA Kit, 192 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Kit Contents Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Quantity 1 Kit(s)
Quantity 192 assays
Species Human
Conjugate Streptavidin-HRP