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HELIOS, FITC, clone: 22F6, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-9883-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15208389

  • £221.14 / 100µg


EU Stock 5
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Description

Description

Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios. Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros. It is expressed in hematopoietic stem cells in both the embryo and adult mice. While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells. Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription. Eight isoforms of Helios exist, some of which possess a dominant-negative function that has also been found to be upregulated in lymphoid malignancies, including T-acute lymphoblastic leukemia.22F6 has been reported to cross react with rat Helios.Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 22F6 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using Foxp3 Fixation and Permeabilization Buffers (cat. 00-5521). Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Helios
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9UKS7, P81183
HELIOS, ZNF1A2, ZNFN1A2, ikaros family zinc finger protein 2, zinc finger DNA binding protein Helios, zinc finger protein Helios, zinc finger protein, subfamily 1A, 2 (Helios)
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
CanineþHumanþMurine
Flow Cytometry
22F6
FITC
IKZF2
Conjugated
Ikzf2
IgG
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
22807
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