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HSP27, clone: STRSN, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9112-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278147

  • £57.93 / 25µg


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For Research Use Only.


Description

Description

Description: The monoclonal antibody STRSN recognizes human heat shock protein 27 (HSP27). The heat shock proteins are a large family of proteins that are induced in response to stressors such as changing temperatures, cytokine levels, and presence of various chemicals. Heat shock proteins serve as molecular chaperones, binding to other proteins to maintain proper protein structure or by folding denatured proteins and preventing activation of caspases that could lead to apoptosis. Unlike the larger HSP proteins, HSP27 belongs to a unique subgroup that is involved in apoptosis regulation. Expression of HSP27 is constitutive, but upon stress HSP27 is upregulated and can relocate from the cytoplasm to the nucleus. Increased HSP27 expression with certain carcinomas has been observed. In breast cancer, HSP27 expression has been shown to be responsive to estrogen levels. In both breast cancer and melanoma, high levels of HSP27 correlate with more aggressive tumors and decreased survival. Increased levels of HSP27 in the serum of patients with Chronic Obstructive Pulmonary Disease (COPD), breast cancer and melanoma may serve as a potential diagnostic marker. No crossreactivity is observed with HSP70, 90, 104 or 110.Applications Reported: This STRSN antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, ELISA, and immunocytochemistry.Applications Tested: This STRSN antibody has been tested by western blot of reduced HeLa cell lysate, immunocytochemistry on fixed and permeabilized A549 cells, and by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human tissue with either low or high pH antigen retreival at less than or equal to 1 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Heat Shock Protein
STRSN
Unconjugated
Purified
Murine
25μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
heat shock protein beta-1, HSPB1, Hsp27
IgG1
RUO
Primary
Human
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