TCR H-Y (male antigen), FITC, clone: T3.70, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 11-9930-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The T3.70 monoclonal antibody reacts with the transgenic alpha betaTCR that recognizes the male antigen H-Y in the context of H-2D^b. A large fraction of T cells in H-Y TCR transgenic mouse expresses this receptor. The H-Y TCR transgenic mouse has been used extensively to study T cell development and the role of thymic major histocompatibility complex in CD4^+ and CD8^+ T cell differentiation.Applications Reported: The T3.70 antibody has been reported for use in flow cytometric analysis.Applications Tested: The T3.70 antibody has been tested by flow cytometric analysis of CD11.3 line, which is derived from a thymic tumor of the H-Y TCR transgenic mice and expresses the transgenic H-Y TCR. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|male antigen HY|
For Research Use Only.