Invitrogen™ GSK-3A Human Protein

Easily screen for kinase binding. Thermo Fisher Invitrogen™ GSK-3A Human Protein recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.

Product Code	Brand	Quantity
15428723	Invitrogen™ PR9188A	100μg
15428813	Invitrogen™ PV6127	1mg

Description

The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.

• Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
• Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody
• No special measures were taken to activate this kinase

GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST reagents.

Specifications

• Accession Number: NP_063937.2
• Formulation: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
• Storage Requirements: Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Structural Form: Full Length
• KinaseFamily: GSK Kinase Family
• Recombinant: Recombinant
• Expression System: Baculovirus

• For Use With (Application): Enzyme and Protein Activity Assay, Kinase Assay, Protein Assays and Analysis, Protein Biology
• Molecular Weight (g/mol): 78.4kDa
• Regulatory Status: RUO - research use only
• Gene Alias: GSK-3A
• Species: Human
• Protein Tag: GST Tag
• Form: Frozen