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GSK-3A Human Protein
GSK-3A Human Protein
Brand: Invitrogen PV6126
Code : 50
Additional Details : Weight : 0.00900kg
Description
This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.
The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).
GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)
This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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Specifications
NP_063937.2 | |
Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
2931 | |
GSK-3A Human Protein | |
–80°C | |
1 Each | |
GSK-3A Human Protein | |
GSK kinase family | |
580nmoles of phosphate transferred to Phospho-Glycogen synthase peptide 2 substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.17μg/mL | |
GST Tag | |
Kinases and inhibitors | |
Serine/threonine kinase |
0.33mg/mL | |
Frozen | |
78.4kDa | |
10μg | |
For research use only. Not for use in diagnostic procedures | |
GSK-3A | |
GSK3A was pre-diluted in enzyme dilution buffer and assayed in 20mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 1mM Na3VO4, 5mM β−glycerophosphate, 2mM DTT, 0.02% Triton™ X-100, 200μM ATP, 50μM Phospho-glycogen synthase peptide 2 substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) and trace [32P]-γ-ATP for 10 minutes at 30°C | |
GSK-3A Human Protein | |
Human | |
Baculovirus | |
Frozen | |
95% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.