missing translation for 'onlineSavingsMsg'
Learn More

GSK-3A Human Protein

GSK-3A Human Protein

Brand:  Invitrogen PV6126

Code : 50

Additional Details : Weight : 0.00900kg

Product Code. 11320872

  • £437.00 / Each
Estimated Shipment: 11-04-2024
to see stock.

Please to purchase this item. Need a web account? Register with us today!


For Research Use Only. All usage must comply with product instructions.
 

Description

Description

This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
  •  Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
  •  Expressed in insect cells
  • GST tagged for use with Eu-labeled GST antibody

    Easily Screen for Kinase Binding
    The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

    The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

    GSK3A for the LanthaScreen Kinase Binding Assay
    Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)

    This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
  •  Tracer 236 (Cat No. PV5592)
  •  Eu-labeled GST Antibody (Cat No. PV5594)

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    Related Link
  •  Learn more about the LanthaScreen™ Eu Binding Assay.
  • Specifications

    Specifications

    NP_063937.2
    Pharma and Biopharma, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay
    2931
    GSK-3A Human Protein
    –80°C
    1 Each
    GSK-3A Human Protein
    GSK kinase family
    580nmoles of phosphate transferred to Phospho-Glycogen synthase peptide 2 substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.17μg/mL
    GST Tag
    Kinases and inhibitors
    Serine/threonine kinase
    0.33mg/mL
    Frozen
    78.4kDa
    10μg
    For research use only. Not for use in diagnostic procedures
    GSK-3A
    GSK3A was pre-diluted in enzyme dilution buffer and assayed in 20mM Tris (pH 7.5), 10mM MgCl2, 1mM EGTA, 1mM Na3VO4, 5mM β−glycerophosphate, 2mM DTT, 0.02% Triton™ X-100, 200μM ATP, 50μM Phospho-glycogen synthase peptide 2 substrate (YRRAAVPPSPSLSRHSSPHQ(pS)EDEEE) and trace [32P]-γ-ATP for 10 minutes at 30°C
    GSK-3A Human Protein
    Human
    Baculovirus
    Frozen
    95% by SDS-PAGE
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    For Research Use Only. Not for use in diagnostic procedures.