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Granzyme M, eFluor 660, clone: 4B2G4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9774-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15510137

  • £223.65 / 100 tests


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Disclaimers:

For Research Use Only.


Description

Description

Description: Granzyme M is one of five granzyme serine proteases that have been indentified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Although not as well characterized as some of the other family members, Granzyme M has been found to exhibit some distinct functions. These include the non-cytotoxic inhibition of cytomegalovirus replication, induction of apoptosis in tumor cells, and contribution to the TLR4-induced inflammatory process.Applications Reported: This 4B2G4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 4B2G4 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Granzyme M
4B2G4
eFluor 660
Conjugated
IgG1
RUO
Primary
Human
Flow Cytometry (Intracellular Staining)
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
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